Phlebia brevispora · Atlas of Contaminated Sites

Phlebia brevispora Nakasone

A white-rot fungus of the genus Phlebia, in which a Japanese group, following phylogeny, found several dioxin degraders. P. brevispora is the first white-rot fungus for which transformation of a recalcitrant tetrachlorodioxin and of coplanar PCBs was demonstrated by identifying the metabolites. A related species, P. radiata, mineralises TNT with its manganese peroxidase.

Contaminants

Contaminant	Evidence	What was shown
Dioxins and furans	laboratory	In culture, 14 days: about 80% of 2,7-dichlorodibenzo-p-dioxin degraded. 1,3,6,8-Tetrachlorodibenzo-p-dioxin, untouched by other Phlebia strains, is transformed into monohydroxy-, monomethoxy- and dimethoxy-tetraCDD, dimethoxy-triCDD and 3,5-dichlorocatechol. Kamei, Suhara & Kondo (2005) Phylogenetical approach to isolation of white-rot fungi capable of degrading polychlorinated dibenzo-p-dioxin. Appl Microbiol Biotechnol 69:358-366, 2005
Polychlorinated biphenyls	laboratory	Mixture of 11 coplanar PCBs (7 mono-ortho and 4 non-ortho) in culture: 5 congeners degraded, including 3,3',4,4'-tetrachlorobiphenyl and 3,3',4,4',5-pentachlorobiphenyl; meta-methoxylated metabolites identified and, for mono-ortho congeners, para-dechlorinated and methoxylated ones. Kamei et al. (2006) Fungal bioconversion of toxic polychlorinated biphenyls by white-rot fungus, Phlebia brevispora. Appl Microbiol Biotechnol 73:932-940, 2006
Explosives (TNT, RDX)	laboratory	Data for Phlebia radiata, not P. brevispora: a cell-free MnP preparation (36 nkat/mL) with 10 mM reduced glutathione transformed 10 mg/L TNT in three days; within 5 days 22% of 14C-TNT and 76% of 14C-2-amino-4,6-dinitrotoluene were mineralised. At 50 mM glutathione degradation is strongly inhibited. Van Aken et al. (1999) Transformation and mineralization of 2,4,6-trinitrotoluene (TNT) by manganese peroxidase from the white-rot basidiomycete Phlebia radiata. Biodegradation 10:83-91, 1999

Contaminant

Evidence

What was shown

Dioxins and furans

laboratory

In culture, 14 days: about 80% of 2,7-dichlorodibenzo-p-dioxin degraded. 1,3,6,8-Tetrachlorodibenzo-p-dioxin, untouched by other Phlebia strains, is transformed into monohydroxy-, monomethoxy- and dimethoxy-tetraCDD, dimethoxy-triCDD and 3,5-dichlorocatechol.

Kamei, Suhara & Kondo (2005) Phylogenetical approach to isolation of white-rot fungi capable of degrading polychlorinated dibenzo-p-dioxin. Appl Microbiol Biotechnol 69:358-366, 2005

Polychlorinated biphenyls

laboratory

Mixture of 11 coplanar PCBs (7 mono-ortho and 4 non-ortho) in culture: 5 congeners degraded, including 3,3',4,4'-tetrachlorobiphenyl and 3,3',4,4',5-pentachlorobiphenyl; meta-methoxylated metabolites identified and, for mono-ortho congeners, para-dechlorinated and methoxylated ones.

Kamei et al. (2006) Fungal bioconversion of toxic polychlorinated biphenyls by white-rot fungus, Phlebia brevispora. Appl Microbiol Biotechnol 73:932-940, 2006

Explosives (TNT, RDX)

laboratory

Data for Phlebia radiata, not P. brevispora: a cell-free MnP preparation (36 nkat/mL) with 10 mM reduced glutathione transformed 10 mg/L TNT in three days; within 5 days 22% of 14C-TNT and 76% of 14C-2-amino-4,6-dinitrotoluene were mineralised. At 50 mM glutathione degradation is strongly inhibited.

Van Aken et al. (1999) Transformation and mineralization of 2,4,6-trinitrotoluene (TNT) by manganese peroxidase from the white-rot basidiomycete Phlebia radiata. Biodegradation 10:83-91, 1999

All data come from pure liquid culture or isolated enzyme. Only 5 of the 11 coplanar PCBs tested are degraded, and the products are methoxylated and hydroxylated derivatives that are still chlorinated: this is transformation, not complete dechlorination. No soil or field trial was found.

Phlebia brevispora Nakasone

How it works

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How it works

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Other species for the same contaminants